Crystallization of Brome Mosaic Virus and T = 1 Brome Mosaic Virus Particles Following a Structural Transition
نویسندگان
چکیده
منابع مشابه
Efficient Amplification of Brome Mosaic Virus
The genome of brome mosaic virus (BMV) is divided among messenger polarity RNA1, RNA2, and RNA3 (3.2, 2.9, and 2.1 kilobases, respectively). cis-Acting sequences required for BMV RNA amplification were investigated with RNA3. By using expressible cDNA clones, deletions were constructed throughout RNA3 and tested in barley protoplasts coinoculated with RNA1 and RNA2. In contrast to requirements ...
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As with transcription from DNA templates, RNA synthesis from viral RNA templates must initiate accurately. RNA sequences named specificity and initiation determinants allow recognition of and coordinated interaction with the viral replication enzyme. Using enriched replicase from brome mosaic virus (BMV)-infected plants and variants of the promoter template for minus-strand and subgenomic RNA i...
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The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protopla...
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Longidorus breviannulatus N o r t o n & Hoffmann, 1975 was described from corn in Iowa. Brome mosaic virus (BMV) was initially isolated in the Great Plains area of the United States from perennial brome grass, Bromus inermis. Infrequent localized outbreaks of BMV in barley were believed to result from mechanical inoculation by farm machinery moving across infected brome grass and into barley fi...
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Packaging of the segmented RNA genome of Brome mosaic virus (BMV) into discrete particles is an essential step in the virus life cycle; however, questions remain regarding the mechanism of RNA packaging and the degree to which the viral coat protein controls the process. In this study, we used a plant-derived glycosidase, Pokeweed antiviral protein, to remove 14 specific bases from BMV RNA3 to ...
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ژورنال
عنوان ژورنال: Virology
سال: 2001
ISSN: 0042-6822
DOI: 10.1006/viro.2000.0897